Research
Research is a fundamental pillar of the Pediatric Hematology and Oncology Unit. Our objective is to improve cure rates and reduce treatment-related toxicity through a deeper understanding of the biological mechanisms underlying childhood cancer and the development of more precise therapeutic strategies.
Research activity integrates advanced molecular studies, development of experimental models, biomarker identification, and multidisciplinary collaboration.
Research Lines
Molecular Characterization of Pediatric Tumors and Identification of Therapeutic Targets
Description: Research aimed at identifying molecular alterations with potential prognostic and therapeutic implications.
Materials and Methods: Next-generation sequencing using commercial gene panels, FISH, PCR, and immunohistochemistry.
Objectives: To define the genomic profiles of pediatric tumors and implement personalized treatments in recurrent or high-risk cases.
Principal Investigator: Dr. López-Ibor
Familial Cancer and Predisposition Syndromes
Description: Study of genetic syndromes associated with predisposition to childhood cancer.
Materials and Methods: Trio exome sequencing in diagnosed patients and their parents.
Objectives: To improve early diagnosis and long-term surveillance of pediatric patients.
Principal Investigators: Dr. Villa and Dr. Camacho
Cancer and Down Syndrome
Description: Investigation of molecular mechanisms underlying the lower incidence of certain solid tumors in patients with Down syndrome.
Materials and Methods: Cell line culture, RNA extraction, qPCR, and microRNA analysis.
Objectives: To identify protective mechanisms and potential therapeutic strategies.
Principal Investigator: Dr. Osuna
Preclinical Models and Patient-Derived Xenografts (PDX)
Description: Study of biological samples to better understand tumor development and evaluate novel therapeutic approaches.
Materials and Methods: Generation of patient-derived xenografts and experimental in vitro and in vivo models.
Objectives: Identification of biomarkers of response and toxicity, as well as new therapeutic targets.
Principal Investigators: Dr. Osuna and Dr. Martín
Biomarkers in Germ Cell Tumors (miRNA 371a-3p)
Description: Multicenter study evaluating the diagnostic and prognostic value of novel molecular biomarkers.
Objectives: To improve diagnosis, monitoring, and follow-up of pediatric patients.
Principal Investigators: Dr. López-Ibor and Dr. Osuna
Cardio-Oncology Research Project
Description: Identification of genetic factors associated with cardiotoxicity in oncologic treatments.
Objectives: To minimize long-term cardiac adverse effects.
Doctoral Theses
Detection of Molecular Markers through Liquid Biopsy in Pediatric Brain Tumors
Author: Dr. Pilar Areal Hidalgo (2022)
Objective: Development of molecular techniques to detect specific mutations in central nervous system tumors.
Methodology: PCR and analysis of tissue, blood, and cerebrospinal fluid samples.
Application: Less invasive tools for tumor monitoring.
Immunolocalization of Substance P and NK-1R Receptor in Adamantinomatous Craniopharyngioma
Author: Dr. Carlos Alcaide (2023)
Objective: To evaluate the potential of NK-1R as a novel therapeutic target.
Differential Protection Against Medulloblastoma in Down Syndrome
Author: Dr. Marta Osuna (ongoing)
Objective: To identify protective molecular mechanisms as a basis for new therapeutic strategies.